Role of NADPH phagocyte oxidase in host defense against acute respiratory Acinetobacter baumannii infection in mice

Download
  1. Get@NRC: Role of NADPH phagocyte oxidase in host defense against acute respiratory Acinetobacter baumannii infection in mice (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1128/IAI.01029-08
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleInfection and Immunity
Volume77
Issue3
Pages10151021; # of pages: 7
SubjectAcinetobacter baumannii; Acinetobacter Infections; Animals; bacterial; Canada; cell; Cells; Cytokines; enzymology; Female; genetics; immunology; Infection; Infections; Lung; metabolism; Mice; Mice,Knockout; Model; Nadph Oxidase; Neutrophils; Nitric Oxide; Nitric Oxide Synthase Type II; pathology; Respiratory Tract Infections; Role; Serum; Spleen
AbstractAcinetobacter baumannii is an emerging bacterial pathogen that rapidly develops multiple-drug resistance and is responsible for many nosocomial pulmonary infections. This study investigated the role of the NADPH phagocyte oxidase (phox) and inducible nitric oxide synthase (NOS2) in the host defense against respiratory infection with A. baumannii in mouse models of intranasal A. baumannii infection. gp91phox⁻/⁻ mice showed higher susceptibility to A. baumannii infection than wild-type (WT) C57BL/6 mice, with significantly greater bacterial counts in their lungs (1,000-fold) (P < 0.005) and spleens (10-fold) (P < 0.05). Moreover, all of the gp91phox⁻/⁻ mice succumbed to infection within 48 h. In contrast, only a moderate increase in bacterial burdens was detected in the lungs of NOS2⁻/⁻ mice, and all NOS2⁻/⁻ mice survived infection. Compared to WT mice, the pulmonary influx of inflammatory cells and serum and local inflammatory cytokine/chemokine responses were not obviously impaired at 4 h and were significantly higher at 24 h (P < 0.05) in gp91phox⁻/⁻ mice, but NADPH-deficient neutrophils were unable to control bacterial replication and extrapulmonary dissemination. Thus, NADPH phagocyte oxidase appears to play a crucial role in the neutrophil-mediated host defense against A. baumannii.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NRC numberQIU2009
NPARC number9372107
Export citationExport as RIS
Report a correctionReport a correction
Record identifiere34b3e46-70d7-4bef-b3c2-a1a0df968bc2
Record created2009-07-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)