Highly stable phospholipid unilamellar vesicles from spontaneous vesiculation : a DLS and SANS study

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DOIResolve DOI: http://doi.org/10.1021/jp047510q
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TypeArticle
Journal titleThe Journal of Physical Chemistry B: Condensed Phase
ISSN15206106
Volume109
Issue1
Pages609616; # of pages: 8
AbstractSpontaneously formed unilamellar vesicles (ULV) composed of short- and long-chain phospholipids, dihexanoyl phosphorylcholine (DHPC) and dimyristoyl phosphorylcholine (DMPC), respectively, were doped with a negatively charged lipid, dimyristoyl phosphorylglycerol (DMPG), and studied with small-angle neutron scattering (SANS) and dynamic light scattering (DLS). Upon dilution, the spontaneous formation of vesicles was found to take place from bilayered micelles, or so-called "bicelles". SANS and DLS data show that ULV with narrow size distributions are highly stable at low lipid (Clp < 0.50 wt %) and NaCl salt (Cs) concentrations. ULV size was found to be independent of both Clp and Cs when they were below 0.33 and 0.5 wt %, respectively. Surface charge and salinity were found to be important factors in preparing ULV of a certain size. This observation is not in complete agreement with previous experimental results and cannot be completely explained with current theoretical predictions based on equilibrium calculations for catanionic surfactant mixtures. ULV size is found to be invariant over a wide range of temperatures, both below and above the phase-transition temperature, TM, of DMPC, and was stable for periods of weeks and months, even after sonication.
Publication date
PublisherAmerican Chemical Society
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedYes
Identifier18077705
NPARC number12332852
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Record identifiere39a317b-2207-400d-8a85-80f321547316
Record created2009-09-10
Record modified2017-03-23
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