Global gene deletion analysis exploring yeast filamentous growth

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DOIResolve DOI: http://doi.org/10.1126/science.1224339
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TypeArticle
Journal titleScience
ISSN0036-8075
Volume337
Issue6100
Pages13521356; # of pages: 5
Subjectgreen fluorescent protein; transcription factor; transcription factor flo8; transcription factor Mss11; unclassified drug; gene expression; genome; growth; immune system; nutrient cycling; polyploidy; yeast; allele; article; biofilm; Candida albicans; cell structure; fungus growth; gene deletion; gene expression; gene identification; haploidy; nonhuman; phenotype; priority journal; promoter region; protein binding; protein localization; Saccharomyces cerevisiae; signal transduction; transcription regulation; Candida albicans; Saccharomyces cerevisiae; Saccharomycetales
AbstractThe dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, S1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute (BRI-IRB)
Peer reviewedYes
NPARC number21269277
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Record identifiere3a0acfa-cd47-47c8-9ede-5f6745a4b8e7
Record created2013-12-12
Record modified2016-05-09
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