Structural studies of the O-chain of the phenol-phase soluble lipopolysaccharide from Haemophilus pleuropneumoniae serotype 2: Biochem.Cell Biol.

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TypeArticle
Journal titleBiochem.Cell Biol.
Volume65
Issue10
Pages876889; # of pages: 14
Subject13C; 1H; analysis; Canada; DEGRADATION; Electrophoresis; Haemophilus; Hydrolysis; isolation & purification; linear; lipid; Lipid A; LIPID-A; LIPOPOLYSACCHARIDE; Lipopolysaccharides; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; Methylation; MOLECULAR; Molecular Conformation; Molecular Weight; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; O-chain; Oxidation-Reduction; Phenol; Phenols; Pleuropneumoniae; POLYSACCHARIDE; REPEATING UNIT; RESONANCE; SEROTYPE; Sodium; STRUCTURAL; structure; UNIT; yield
AbstractThe phenol-phase soluble cellular lipopolysaccharide that was isolated by the phenol-water extraction from Haemophilus pleuropneumoniae serotype 2 was shown to be of the S type by sodium dodecyl sulfate--polyacrylamide gel electrophoresis, hydrolysis, methylation, specific degradations, and both one- and two-dimensional 1H and 13C nuclear magnetic resonance studies. It could be cleaved to yield a lipid A and an O-chain polysaccharide. This O-polysaccharide was identified as a high molecular weight unbranched linear polymer of a pentasaccharide repeating unit having the structure: (Formula: see text)
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LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberALTMAN1987G
NPARC number9371269
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Record identifiere4e49b3c-2631-4e4e-929d-e72ad2da89ec
Record created2009-07-10
Record modified2016-05-09
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