Application of isothermal titration calorimetry for characterizing thermodynamic parameters of biomolecular interactions : peptide self-assembly and protein adsorption case studies

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DOIResolve DOI: http://doi.org/10.1021/bm5004515
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TypeArticle
Journal titleBiomacromolecules
ISSN1525-7797
Volume15
Issue10
Pages34633473; # of pages: 11
SubjectBiomolecular interactions; Isothermal titration calorimetry; Peptide self assemblies; Protein adsorption; Thermodynamic parameter; peptide; polymer; protein; adiabaticity; adsorption; Article; case study; enthalpy; entropy; equilibrium constant; human; isothermal titration calorimetry; macromolecule; molecular interaction; protein analysis; surface plasmon resonance; theory; thermodynamics
AbstractThe complex nature of macromolecular interactions usually makes it very hard to identify the molecular-level mechanisms that ultimately dictate the result of these interactions. This is especially evident in the case of biological systems, where the complex interaction of molecules in various situations may be responsible for driving biomolecular interactions themselves but also has a broader effect at the cell and/or tissue level. This review will endeavor to further the understanding of biomolecular interactions utilizing the isothermal titration calorimetry (ITC) technique for thermodynamic characterization of two extremely important biomaterial systems, viz., peptide self-assembly and nonfouling polymer-modified surfaces. The advantages and shortcomings of this technique will be presented along with a thorough review of the recent application of ITC to these two areas. Furthermore, the controversies associated with the enthalpy-entropy compensation effect as well as thermodynamic equilibrium state for such interactions will be discussed.
Publication date
LanguageEnglish
AffiliationSecurity and Disruptive Technologies; National Research Council Canada
Peer reviewedYes
NPARC number21272657
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Record identifiere55f80fd-9f1d-4686-9b90-407f8edc2cf8
Record created2014-12-03
Record modified2016-05-09
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