A hexane fraction of American ginseng suppresses mouse colitis and associated colon cancer: Anti-inflammatory and proapoptotic mechanisms

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DOIResolve DOI: http://doi.org/10.1158/1940-6207.CAPR-11-0421
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleCancer Prevention Research
ISSN1940-6207
Volume5
Issue4
Pages685696; # of pages: 12
Subjectacetic acid ethyl ester; azoxymethane; butanol; dextran sulfate; dichloromethane; ginseng extract; hexane; water; antiinflammatory agent; plant extract; animal cell; antiinflammatory activity; antineoplastic activity; antioxidant activity; article; colitis; colon cancer; controlled study; ginseng; human; human cell; in vitro study; lymphoblastoid cell line; macrophage; nick end labeling; nonhuman; priority journal; animal; apoptosis; bioassay; chemistry; colon tumor; inflammation; mass fragmentography; metabolism; methodology; mouse; Panax; tumor cell line; ulcerative colitis; Animals; Anti-Inflammatory Agents; Apoptosis; Biological Assay; Cell Line, Tumor; Colitis, Ulcerative; Colonic Neoplasms; Gas Chromatography-Mass Spectrometry; Hexanes; Humans; In Situ Nick-End Labeling; Inflammation; Mice; Panax; Plant Extracts
AbstractUlcerative colitis is a chronic inflammatory condition associated with a high colon cancer risk. We have previously reported that American ginseng extract significantly reduced the inflammatory parameters of chemically induced colitis. The aim of this study was to further delineate the components of American ginseng that suppress colitis and prevent colon cancer. Among five different fractions of American ginseng (butanol, hexane, ethylacetate, dichloromethane, and water), a hexane fraction has particularly potent antioxidant and proapoptotic properties. The effects of this fraction were shown in a mouse macrophage cell line (ANA-1 cells), in a human lymphoblastoid cell line (TK6), and in an ex vivo model (CD4 +/CD25 - primary effector T cells). A key in vivo finding was that compared with the whole American ginseng extract, the hexane fraction of American ginseng was more potent in treating colitis in a dextran sodium sulfate (DSS) mouse model, as well as suppressing azoxymethane/DSS-induced colon cancer. Furthermore, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) labeling of inflammatory cells within the colonic mesenteric lymph nodes was elevated in mice consuming DSS + the hexane fraction of American ginseng. Results are consistent with our in vitro data and with the hypothesis that the hexane fraction of American ginseng has anti-inflammatory properties and drives inflammatory cell apoptosis in vivo, providing a mechanism by which this fraction protects from colitis in this DSS mouse model. This study moves us closer to understanding the molecular components of American ginseng that suppress colitis and prevent colon cancer associated with colitis. ©2012 AACR.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for National Measurement Standards (INMS-IENM)
Peer reviewedYes
NPARC number21269351
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Record identifiere5609b86-9ac9-477e-ba7a-268e08051c65
Record created2013-12-12
Record modified2016-05-09
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