Quantitative determination of apoptosis on leukemia cells by infrared spectroscopy

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DOIResolve DOI: http://doi.org/10.1023/A:1011383408381
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TypeArticle
Journal titleApoptosis
ISSN1360-8185
1573-675X
Volume6
Issue4
Pages269278; # of pages: 10
Subjectapoptosis; leukemic cells; infrared spectroscopy; molecular changes; quantitative analysis
AbstractQuantitation of apoptotic cell death in vivo has become an important issue for patients with acute leukemia. We describe herein a new analytical method, based on infrared (IR) spectroscopy, to estimate the percentage of apoptotic leukemic cells in two different cell lines (CEM and K562), induced with etoposide (VP-16). As the percentage of apoptosis increases, the protein structure shifts from dominantly β-sheet to unordered (random coil), the overall lipid content increases and the amount of detectable DNA decreases. These changes can be directly related to the percentage of apoptosis as determined by two standard reference methods: flow cytometry and DNA ladder formation. The correlation between the significant IR spectral changes and the percentage of apoptotic leukemia cells in the two cell lines was optimal up to 24 h following etoposide treatment (r = 0.99 for CEM cells and r = 0.96 for K562 cells). Furthermore, IR spectroscopy is able to detect apoptotic changes in these cells already after 4 h treatment with VP-16, compared to flow cytometry which needs 6 h to observe significant changes. Our study suggests that IR spectroscopy may have potential clinical utility for the early, fast and reagent free assessment of chemotherapeutic efficacy in patients with leukemia.
Publication date
PublisherKluwer Academic Publishers
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NRC number1887
NPARC number9148380
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Record identifiere624100e-ed47-4629-9e4e-620dc5c63dc8
Record created2009-06-25
Record modified2016-09-29
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