Probing cytotoxicity of nanoparticles and organic compounds using scanning proton microscopy, scanning electron microscopy and fluorescence microscopy

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DOIResolve DOI: http://doi.org/10.1016/j.nimb.2008.09.006
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TypeArticle
Journal titleNuclear Instruments and Methods in Physics Research B
Volume266
Issue23
Pages50415046; # of pages: 6
SubjectENV; calcium ion; ethidium bromide; benzo[a]pyrene; cytotoxicity; scanning proton microscopy
AbstractScanning proton microscopy, scanning electron microscopy (SEM) and fluorescence microscopy have been used to probe the cytotoxicity effect of benzo[a]pyrene (BaP), ethidium bromide (EB) and nanoparticles (ZnO, Al₂O₃ and TiO₂) on a T lymphoblastic leukemia Jurkat cell line. The increased calcium ion (from CaCl2) in the culture medium stimulated the accumulation of BaP and EB inside the cell, leading to cell death. ZnO, Al₂O₃ and TiO₂ nanoparticles, however, showed a protective effect against these two organic compounds. Such inorganic nanoparticles complexed with BaP or EB which became less toxic to the cell. Fe₂O₃ nanoparticles as an insoluble particle model scavenged by macrophage were investigated in rats. They were scavenged out of the lung tissue about 48 h after infection. This result suggest that some insoluble inorganic nanoparticles of PM (particulate matters) showed protective effects on organic toxins induced acute toxic effects as they can be scavenged by macrophage cells. Whereas, some inorganic ions such as calcium ion in PM may help environmental organic toxins to penetrate cell membrane and induce higher toxic effect.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number49983
NPARC number12440527
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Record identifiere6703690-3e30-459e-9b23-676e142aad9a
Record created2009-10-26
Record modified2016-05-09
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