Electrochemically-assisted deposition of oxidases on platinum nanoparticle/multi-walled carbon nanotube-modified electrodes

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TypeArticle
Journal titleThe Analyst
Volume132
Issue12
Pages12541261; # of pages: 8
Subjectanalysis; Carbon; env; Enzymes; Glucose; Hydrogen Peroxide; Oxygen; Precipitation
AbstractPlatinum nanoparticles were electrodeposited by a multi-potential step technique onto a multi-walled carbon nanotube (MWCNT) film pre-casted on a glassy carbon (GC) or boron-doped diamond (BDD) electrode. The MWCNT network consisted of Pt nanoparticles with an average diameter of 120 nm after an optimization of 36 deposition cycles. The resulting electrochemical sensors were capable of detecting hydrogen peroxide as low as 25 nM. Five different enzymes: glucose, lactate, glutamate, amino acid and xanthine oxidases, respectively, were deposited by a constant current technique for 5�10 min to form a stable and active biolayer for the analysis of their corresponding analytes. The glucose oxidase-based biosensor was linear up to 10 mM glucose with a detection limit of 250 nM and a response time of 5 s. Similar response times and detection limits were observed with glutamate, lactate, and amino acid oxidase despite the fact that the linear ranges were noticeably narrower. The mechanism of deposition was attributed to the decrease of local pH, created by oxygen evolution and effected enzyme precipitation.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number49087
NPARC number3539268
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Record identifiere71dff81-5389-4ace-96c5-ff42d089856a
Record created2009-03-01
Record modified2016-05-09
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