Analysis of expressed sequence tags in Brassica napus cotyledons damaged by crucifer flea beetle feeding

Alternative titleAnalysis of sequence tags in Brassica cotyledons damaged by crucifer flea beetle feeding
Download
  1. Get@NRC: Analysis of expressed sequence tags in Brassica napus cotyledons damaged by crucifer flea beetle feeding (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1139/g11-083
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleGenome
ISSN0831-2796
1480-3321
Volume55
Issue2
Pages118133; # of pages: 16
SubjectBrassica napus; cotyledon EST representation; flea beetle Phyllotreta; host plant molecular response
AbstractThe molecular basis of canola (Brassica napus L.) susceptibility to the crucifer flea beetle (FB, Phyllotreta cruciferae Goeze) was investigated by comparing transcript representation in FB-damaged and undamaged cotyledons. The B. napus cotyledon transcriptome increased and diversified substantially after FB feeding damage. Twenty-two genes encoding proteins with unknown function, six encoding proteins involved in signaling, and a gene encoding a B-box zinc finger transcription factor were moderately or strongly changed in representation with FB feeding damage. Zinc finger and calciumdependent genes formed the largest portion of transcription factors and signaling factors with changes in representation. Six genes with unknown function, one transcription factor, and one signaling gene specific to the FB-damaged library were co-represented in a FB-damaged leaf library. Out of 188 transcription factor and signaling gene families screened for “early” expression changes, 16 showed changes in expression within 8 h. Four of these early factors were zinc finger genes with representation only in the FB-damaged cotyledon. These genes are now available to test their potential at initiating or specifying cotyledon responses to crucifer FB feeding.
Publication date
PublisherNational Research Council Canada. Research Press
LanguageEnglish
AffiliationNational Research Council Canada; NRC Plant Biotechnology Institute
Peer reviewedYes
NRC number54632
NPARC number21268458
Export citationExport as RIS
Report a correctionReport a correction
Record identifiere795a846-b518-4116-af47-b3f8b8e18ee6
Record created2013-07-24
Record modified2016-10-04
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)