Rapid detection of microorganisms with nanoparticles and electron microscopy

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DOIResolve DOI: http://doi.org/10.1002/jemt.20614
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TypeArticle
Journal titleMicroscopy Research and Technique
Volume71
Issue10
Pages742748; # of pages: 7
SubjectENV; Escherichia coli; Rhodococcus rhodochrous; Candida sp.; electrostatic interactions
AbstractRapid detection of microorganisms is highly desirable. A procedure has been developed based on interactions between gold nanoparticles and proteins of microorganisms (Escherichia coli, Rhodococcus rhodochrous, and Candida sp.) followed by scanning electron microscopy (SEM). The nanoparticle-cell interaction was confirmed by ultraviolet resonance Raman spectroscopy (UVRS) in the SEM focus. Cell suspensions in a buffer were interacted with gold nanoparticles (<10 nm in diameter) prepared from tetrachloroauric acid and sodium borohydride. Possible interference of elevated salt concentrations was eliminated by dialysis in deionized water. Small (10 µL) aliquots of cell-nanoparticle suspensions were dried on a silicon wafer and photographed under an SEM. Characteristic bacterial or yeast cell images in the micrographs indicated the actual presence of microorganisms in the suspension examined. This was further confirmed by UV resonance Raman spectroscopy.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number49942
NPARC number12473134
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Record identifiere7be1ee4-9ee2-4c03-9319-e6703fb218a9
Record created2009-10-26
Record modified2016-05-09
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