Analysis of Phytohormone profiles during male and female cone initiation and early differentiation in long-shoot buds of Lodgepole pine

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DOIResolve DOI: http://doi.org/10.1007/s00344-011-9257-1
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TypeArticle
Journal titleJournal of Plant Growth Regulation
ISSN0721-7595
1435-8107
Volume31
Issue4
Pages478489; # of pages: 12
Subjectcone gender; Lodgepole pine; long-shoot bud; genotype; metabolite; phytohormone
AbstractIn lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia Engelm), cone initiation and gender differentiation are site-specific in long-shoot buds with female cones in the distal portion and male cones in the proximal portion. By using high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) in multiple reaction monitoring (MRM) mode, cytokinins, indole-3-acetic acid (IAA), gibberellins (GAs), abscisic acid (ABA) and their selected metabolites, were investigated in developing long-shoot buds from multiple genotypes. Spatially, higher concentrations of trans-zeatin riboside (t-ZR) and dihydrozeatin (dhZR) existed in the distal parts of long-shoot buds, whereas concentrations of isopentenyl adenosine (iPA), IAA, GA24, ABA, ABA glucose ester (ABA-GE) and phaseic acid (PA) were higher in the proximal parts in all investigated genotypes. In long-shoot buds of genotypes with a history of high female cone yield, concentrations of t-ZR and the ratio of zeatin-type to isopentenyl-type cytokinins were higher in the entire buds, whereas dhZR or IAA was higher either in the distal or in the proximal part respectively. In low female cone yielding genotypes, concentrations of c-ZR, iPA, ABA-GE and PA were higher in both of the parts, whereas ABA was higher mainly in the distal part with higher GA24 in the proximal part. Temporally, concentrations of several hormone-related compounds showed obvious changes in late June and late July, prior to male and female cone bud differentiation. This study reveals that the local hormonal status in a long-shoot bud at specific developmental stages may play an important role in gender determination and cone yield.
Publication date
PublisherSpringer
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NRC number54627
NPARC number19559829
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Record identifiere80eabcd-98d6-4287-bdd8-d87e85481076
Record created2012-04-02
Record modified2016-05-09
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