Spirolide production and photoperiod-dependent growth of the marine dinoflagellate Alexandrium ostenfeldii

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TypeArticle
Proceedings titleProceedings of the Ninth International Conference on Harmful Algal Blooms, Hobart, Australia, Feb. 7-11, 2000
ConferenceThe Ninth International Conference on Harmful Algal Blooms, Feb. 7-11, 2000, Hobart, Australia
Pages299302
AbstractThe effects of physiological status on spirolide production were studied in nutrient-replete batch cultures of a toxic strain of the dinoflagellate Alexandrium ostenfeldii. Although complete ce11 synchronisation was not achieved by dark adaptation, the concentration of motile vegetative cells apparently increased in the light anddecreased in the dark. The concentration of extracted chlorophyll a followed the same trend as the ce11 concentration, with no apparent shift in the amount of chlorophyll a per ce11 in relation to the light/dark (L/D) phase. Analysis of spirolides by liquid chromatography coupled with mass spectrometry (LC-MS) showed that the toxin profile did not vary significantly over the L/D cycle, and consisted primarily of a des-methyl-C derivative (>90% molar), with minor constituents C, C3, D, D3 and des-methyl-D. The total spirolide concentration per unit culture volume was directly related to the concentration of cells and chlorophyll a, but there was a dramatic increase in ce11 quota of spirolides at the beginning of the dark phase and a corresponding decrease in the light. The biosynthesis of these polyketide-derived metabolites is apparently govemed by light-dependent events during the ce11 division cycle.
Publication date
PublisherInternational Oceanographic Commission (UNESCO)
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NPARC number23001117
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Record identifiere8f08d49-b40e-4f43-9130-6bef5834939b
Record created2016-12-13
Record modified2016-12-13
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