Microstructure of Anaerobic Granules Bioaugmented with Desulfitobacterium frappieri PCP-1

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DOIResolve DOI: http://doi.org/10.1128/AEM.68.8.4035-4043.2002
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TypeArticle
Journal titleAcoustics, Speech, and Signal Processing Newsletter, IEEE
Volume68
Issue8
Pages40354043; # of pages: 9
AbstractOligonucleotide probes were used to study the structure of anaerobic granular biofilm originating from a pentachlorophenol-fed upflow anaerobic sludge bed reactor augmented with Desulfitobacterium frappieri PCP-1. Fluorescence in situ hybridization demonstrated successful colonization of anaerobic granules by strain PCP-1. Scattered microcolonies of strain PCP-1 were detected on the biofilm surface after 3 weeks of reactor operation, and a dense outer layer of strain PCP-1 was observed after 9 weeks. Hybridization with probes specific for Eubacteria and Archaea probes showed that Eubacteria predominantly colonized the outer layer, while Archaea were observed in the granule interior. Mathematical simulations showed a distribution similar to that observed experimentally when using a specific growth rate of 2.2 day-1 and a low bacterial diffusion of 10-7 dm2 day-1. Also, the simulations showed that strain PCP-1 proliferation in the outer biofilm layer provided excellent protection of the biofilm from pentachlorophenol toxicity.
Publication date
AffiliationNational Research Council Canada; NRC Industrial Materials Institute; NRC Biotechnology Research Institute
Peer reviewedNo
NPARC number12339253
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Record identifiere8f40feb-20f9-41e6-9053-385bca6e3c96
Record created2009-09-11
Record modified2016-05-09
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