Transcriptional response of Choristoneura fumiferana to sublethal exposure of Cry1Ab protoxin from Bacillus thuringiensis

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DOIResolve DOI: http://doi.org/10.1111/j.1365-2583.2006.00659.x
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TypeArticle
Journal titleInsect Molecular Biology
Volume15
Issue4
Pages475483; # of pages: 9
Subjectenv; Insects; Polymerase Chain Reaction; Genes; Bacillus thuringiensis; Christoneura fumiferana; subtractive hybridization; gene expression
AbstractBacillus thuringiensis is a microbial control agent active against Choristoneura fumiferana, a lepidopteran defoliator of North American forests. Although the B. thuringiensis insecticidal crystal protoxins have a relatively narrow host range, there is concern about their impact on non-target species where intoxication effects may not be overt. Larval toxicity effects can be assessed at the molecular level by determining altered transcriptional profiles in response to sublethal protoxin exposure in sensitive insects. Subtraction hybridization libraries were created using two larval populations, control and protoxin-fed and were characterized by sequencing 1091 clones. Differential mRNA expression of selected clones, as measured by quantitative polymerase chain reaction, identified a number of metabolic and stress-related genes that were either transcriptionally enhanced or repressed after protoxin exposure.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number49013
NPARC number3540262
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Record identifiere982f9db-2dc6-43b6-bc96-e2870d032adc
Record created2009-03-01
Record modified2016-05-09
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