On-line monitoring of the progress of infection in Sf-9 insect cell cultures using relative permittivity measurements

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TypeArticle
Journal titleBiotechnology and Bioengineering
Volume63
Issue1
Pages122126; # of pages: 5
Subjectcapacitance; permittivity; on-line monitoring; baculovirus; insect cells
AbstractThe use of on-line relative permittivity (ϵ′) measurements for monitoring cultures of Sf-9 cells was evaluated in a batch culture and a batch infected with a baculovirus expressing β-galactosidase. It was found that viable cell density and volume essentially accounted for all the variation in ϵ′ in both non-infected and synchronously infected cultures, indicating that the ϵ′ of a cell suspension was sensitive only to changes in the viable cell population. Additionally the parameter provided clearly defined signposts of the progress of the infection.
Publication date
Linkhttp://onlinelibrary.wiley.com/doi/10.1002/(SICI)1097-0290(19990405)63:1%3C122::AID-BIT13%3E3.0.CO;2-I/abstract
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number37675
NPARC number3538808
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Record identifiereb4b2a13-4eee-4bca-94ac-cc366ff27e74
Record created2009-03-01
Record modified2016-05-09
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