Sensitive Amperometric Immunosensing Using Polypyrrolepropylic Acid Films for Biomolecule Immobilization

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DOIResolve DOI: http://doi.org/10.1021/ac060657o
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TypeArticle
Journal titleAnalytical Chemistry
Volume78
Issue21
Pages74247431; # of pages: 8
Subjectenv
AbstractAn electrochemical immunosensor was constructed using an electropolymerized pyrrolepropylic acid (PPA) film with high porosity and hydrophilicity. A high density of carboxyl groups of PPA was used to covalently attach protein probes, leading to significantly improved detection sensitivity compared with conventional entrapment methods. As a model, anti-mouse IgG was covalently immobilized or entrapped in the PPA film and used in a sandwich-type alkaline phosphatase-catalyzing amperometric immunoassay with p-aminophenyl phosphate as the substrate. With covalent binding, the detection limit for IgG in PBS buffer, pH 7.4, was 100 pg/mL with a dynamic range of 5 orders of magnitude. The covalent bonding mode in the carbonate-bicarbonate buffer, pH 9.6, further brought down the detection limit to 20 pg/mL with remarkable selectivity.
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AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number49019
NPARC number21275949
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Record identifierebd69862-f15e-4fd6-9ffa-8a2b0870edf5
Record created2015-08-13
Record modified2016-05-09
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