Single-domain antibody functionalized CdSe/ZnS quantum dots for cellular imaging of cancer cells

Download
  1. Get@NRC: Single-domain antibody functionalized CdSe/ZnS quantum dots for cellular imaging of cancer cells (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/jp809159k
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Physical Chemistry C
Volume113
Issue2
Pages496499; # of pages: 4
SubjectPhotoluminescent (PL) semiconductor nanocrystals
AbstractPhotoluminescent (PL) semiconductor nanocrystals, when spherical in shape also termed as quantum dots (QDs), have attracted significant attention in biolabeling and bioimaging applications. Usually, such biooriented applications require targeting to the site of interest, and the use of antibodies is acknowledged as one common strategy for specific and compelling targeting. Conventional antibodies and some of their derivatives have been tested as targeting agents; to the best of our knowledge, the present study is the first with the use of single-domain antibodies (sdAbs) to overcome some disadvantages related to issues such as stability, aggregation, and production cost. Our sdAbs, which are small but fully functional recognition proteins and derived from camelid species, are superior to all of the above antibody choices. This manuscript addresses our efforts on the synthesis and targeting of bioconjugated PL QDs with a sdAb named EG2, which binds strongly to epidermal growth factor receptor (EGFR), a protein of which is widely known as a tumor marker. PEGylation was performed at the same time. The entity of our PEGylated-and-sdAb-conjugated QDs, presented as proof of principle, is robust in specific labeling of EGFR on in vitro grown SK-BR3 and MDA-MB468 human breast-cancer cells.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences; NRC Steacie Institute for Molecular Sciences
Peer reviewedYes
NPARC number15314093
Export citationExport as RIS
Report a correctionReport a correction
Record identifierec0d7281-4ea0-48da-b7a5-064b661aec9d
Record created2010-05-17
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)