Porous graphitized carbon monolith as an electrode material for probing direct bioelectrochemistry and selective detection of hydrogen peroxide

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DOIResolve DOI: http://doi.org/10.1021/ac203061m
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TypeArticle
Journal titleAnalytical Chemistry
ISSN0003-2700
1520-6882
Volume84
Issue5
Pages23512357; # of pages: 7
AbstractFor the first time, graphitized carbon particles with a high surface area have been prepared and evaluated as a new material for probing direct electrochemistry of hemoglobin (Hb). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) imaging revealed that the carbon monolithic skeleton was constructed by a series of mesopores with irregular shapes and an average pore diameter of 5.6 nm. With a surface area of 239.6 m2/g, carbon particles exhibited three major Raman peaks as commonly observed for carbon nanotubes and other carbon materials, i.e., the sp3 and sp2 carbon phases coexisted in the sample. A glassy carbon electrode modified with carbon monoliths and didodecyldimethylammonium bromide exhibited direct electron transfer between Hb molecules and the underlying electrode with a transfer rate constant of 6.87 s–1. The enzyme electrode displayed a pair of quasi-reversible reduction–oxidation peaks at −0.128 and −0.180 V, reflecting the well-known feature of the heme [Fe3+/Fe2+] redox couple: a surface-controlled electrochemical process with one electron transfer. This reagentless biosensing approach was capable of detecting H2O2, a simple molecule but plays an important role in analytical and biological chemistry, as low as 0.1 μM with linearity of 0.1–60 μM and a response time of <0.8 s, comparing favorably with other carbon based electrodes (5 s).
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NPARC number21268855
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Record identifierec367255-8b20-4d69-9846-60e5a3a5656c
Record created2013-11-15
Record modified2016-05-09
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