Study of chronic lymphocytic leukemia cells by FT-IR spectroscopy and cluster analysis

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DOIResolve DOI: http://doi.org/10.1016/0145-2126(96)00019-7
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TypeArticle
Journal titleLeukemia Research
ISSN0145-2126
Volume20
IssueAugust 8
Pages649655; # of pages: 8
SubjectFT-IR spectroscopy; chronic lymphocytic leukemia; cluster analysis; B-Lymphocyte; Fourier spectrometry; Lipids; Proteins; Chronic; Malignant hemopathy; Lymphoproliferative syndrome
AbstractThe peripheral mononuclear cells from 23 normal individuals and the purified B cells from 38 patients with chronic lymphocytic leukemia (CLL) were examined by Fourier transform infrared (FT-IR) spectroscopy. Differences were observed between the CLL and normal cells at the DMA, protein and lipid levels, with CLL cells having greater DNA and lower lipid contents than normal cells. In addition, the spectral character of the CLL and normal cells varied, demonstrating that there were also qualitative differences in the DNA and lipids. Statistical analysis, based on hierarchical clustering, separated normal from CLL cells completely and classified them into two subgroups for normal cells, while the CLL cells could be divided into three subgroups that were distinct from the normal cells. These differences were based on the lipid and DNA content and the overall spectral character of the cells.
Publication date
PublisherElsevier B.V.
Copyright noticeYou may reproduce (print, make photocopies, or download) materials from NPArC without permission for non-commercial purposes (research, education, and private study), on the condition that you provide proper attribution of the sources in all copies.
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics (IBD-IBD); National Research Council Canada
Access conditionavailable
unlimited
public
Peer reviewedYes
NRC number415
NPARC number9201851
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Record identifiered05b69f-f63c-4e6a-a077-25b97a2781e5
Record created2009-10-02
Record modified2016-11-15
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