Naturally-occurring human serum antibodies to inner core lipopolysaccharide epitopes of Neisseria meningitidis protect against invasive meningococcal disease caused by isolates displaying homologous inner core structures

Download
  1. Get@NRC: Naturally-occurring human serum antibodies to inner core lipopolysaccharide epitopes of Neisseria meningitidis protect against invasive meningococcal disease caused by isolates displaying homologous inner core structures (Opens in a new window)
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleVaccine
Volume26
Issue51
Pages66556663; # of pages: 9
SubjectAdolescent; Adult; Age Factors; Aged; Animals; Antibodies; Antibodies,Bacterial; antibody; backbone; bacterial; Blood; Child,Preschool; CORE REGION; DISEASE; Enzyme-Linked Immunosorbent Assay; epitope; Epitopes; Flow Cytometry; Human; Humans; Immunoglobulin G; IMMUNOGLOBULIN-G; immunology; In Vitro; Infant; INNER CORE REGION; isolation & purification; LIPOPOLYSACCHARIDE; Lipopolysaccharides; LPS; Meningococcal Infections; Middle Aged; MUTANT; Neisseria; Neisseria meningitidis; prevention & control; Rats; REGION; RESIDUES; Serum; Serum Bactericidal Test; STRAIN; structure; surface; Young Adult
AbstractSera from healthy infants (under 1 year old), toddlers (3-4 years) and adults (18-65 years) were assayed for their ability to bind to inner core (ic) lipopolysaccharide (LPS) epitopes of Neisseria meningitidis. Antibodies (Abs) reacting to inner core structures, including different substitutions of the first heptose (HepI) and second heptose (HepII) residues of the LPS backbone, truncated and fully extended LPS glycoforms, were detected and for each structure, these inner core antibodies showed an age-related pattern of acquisition. A novel column-based methodology was used to affinity purify IgG antibodies in which purified inner core LPS (derived from a mutant MC58) was covalently linked to Sepharose 4B. Comparison of reactivity before and after affinity purification of the pooled sera showed that the purified Abs bound to the surface of N. meningitidis organisms displaying truncated and extended LPS with a homologous inner core region, promoted the deposition of C3b, were opsonophagocytic in vitro and decreased bacteraemia when used to passively protect infants rats. In addition, the purified Abs were bactericidal in vitro against the mutant strain displaying truncated LPS with a homologous inner core region. These results demonstrate that naturally occurring serum human antibodies to N. meningitidis LPS can access inner core epitopes of encapsulated organisms with a fully extended LPS
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberJAKEL2008
NPARC number9370180
Export citationExport as RIS
Report a correctionReport a correction
Record identifiered5161a4-6830-49bf-af62-424f1ff90e43
Record created2009-07-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)