Activation of innate immune responses by Haemophilus influenzae lipooligosaccharide

  1. Get@NRC: Activation of innate immune responses by Haemophilus influenzae lipooligosaccharide (Opens in a new window)
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Journal titleClinical and Vaccine Immunology
Pages769776; # of pages: 8
Subjectcaspase recruitment domain protein 15; caspase recruitment domain protein 4; CD40 antigen; CD86 antigen; HLA A antigen; HLA B antigen; HLA C antigen; HLA DR antigen; intercellular adhesion molecule 1; interleukin 10; interleukin 1beta; lipid A; lipooligosaccharide; lymphocyte function associated antigen 3; pattern recognition receptor; toll like receptor 4; tumor necrosis factor alpha; adaptive immunity; antigen presentation; article; bacterial strain; controlled study; cytokine response; Escherichia coli; gene expression; Haemophilus influenzae; human; human cell; immunostimulation; innate immunity; matrix assisted laser desorption ionization time of flight mass spectrometry; monocyte; nonhuman; priority journal; protein expression; upregulation
AbstractA Gram-negative pathogen Haemophilus influenzae has a truncated endotoxin known as lipooligosaccharide (LOS). Recent studies on H. influenzae LOS highlighted its structural and compositional implications for bacterial virulence; however, the role of LOS in the activation of innate and adaptive immunity is poorly understood. THP-1 monocytes were stimulated with either lipopolysaccharide (LPS) from Escherichia coli or LOS compounds derived from H. influenzae Eagan, Rd, and Rd lic1 lpsA strains. Cell surface expression of key antigen-presenting, costimulatory, and adhesion molecules, as well as gene expression of some cytokines and pattern recognition receptors, were studied. Eagan and Rd LOS had a lower capacity to induce the expression of ICAM-1, CD40, CD58, tumor necrosis factor alpha (TNF-α), and interleukin-1β (IL-1β) compared to LPS. In contrast, antigen-presenting (HLA-ABC or HLA-DR) and costimulatory (CD86) molecules and NOD2 were similarly upregulated in response to LOS and LPS. LOS from a mutant Rd strain (Rd lic1 lpsA) consistently induced higher expression of innate immune molecules than the wild-type LOS, suggesting the importance of phosphorylcholine and/or oligosaccharide extension in cellular responses to LOS. An LOS compound with a strong ability to upregulate antigen-presenting and costimulatory molecules combined with a low proinflammatory activity may be considered a vaccine candidate to immunize against H. influenzae. Copyright © 2014, American Society for Microbiology. All Rights Reserved.
Publication date
AffiliationNational Research Council Canada (NRC-CNRC); Human Health Therapeutics (HHT-TSH)
Peer reviewedYes
NPARC number21272233
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Record identifiered589c44-df9a-45be-81e0-9fec669540b0
Record created2014-07-23
Record modified2016-05-09
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