The use and misuse of FTIR spectroscopy in the determination of protein structure

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DOIResolve DOI: http://doi.org/10.3109/10409239509085140
AuthorSearch for: ; Search for:
TypeArticle
Journal titleCritical Reviews in Biochemistry and Molecular Biology
ISSN1040-9238
1549-7798
Volume30
Issue2
Pages95120; # of pages: 26
Subjectinfrared spectroscopy; proteins; secondary structure; quantitation; IT
AbstractFourier transform infrartd (FTIR) spectroscopy is an established tool for the structural characterization of proteins. However, many potential pitfalls exist for the unwary investigator. In this review we critically assess the application of FIlR spectroscopy to the determination of protein structure by (1) outlining the principles underlying protein secondary structure determination by FZZR spectroscopy. (2) highhghting the situations in which FZZR spectroscopy should be considered the technique of choice, (3) discussing the manner in which experiments should be conducted to derive as much physiologically relevant information as possible, and (4) outlining current methods for the determination of secondary structure from infrared spectm of proteins.
Publication date
PublisherInforma UK Limited
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NRC number173
NPARC number9148110
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Record identifierede2bf1b-4314-45b2-b7ea-28eef2a9e98e
Record created2009-06-25
Record modified2016-12-02
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