Identification and characterization of protein glycosylation on bacterial flagellin

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TypeArticle
AbstractThe structural characterization of O-linked glycans present on bacterial flagellins was analyzed. The modified peptides were identified by combination of enzymatic cleavage and LC/MS analysis. The flagellins were digested by trypsin which were analyzed using liquid chromatography coupled to nanoelectrospray mass spectrometry on Q-TOF instrument. The nanoLC/MS-MS experiment provided peptide sequence coverage map of 96% and glycosylation site was identified using alkaline hydrolysis.
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LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NPARC number21277189
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Record identifieree6b27bd-5f76-476b-a072-9b5f5a0ea7eb
Record created2016-01-05
Record modified2016-05-09
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