Quantitation of glucose and urea in whole blood by mid-infrared spectroscopy of dry films

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DOIResolve DOI: http://doi.org/10.1016/S0924-2031(01)00150-3
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TypeArticle
Journal titleVibrational Spectroscopy
Volume28
Issue1
Pages111116; # of pages: 6
SubjectBlood analysis; Infrared spectroscopy; PLS; Whole blood; Glucose; Urea; Clinical analysis
AbstractThis paper reports the results of a study to determine whether mid-infrared (IR) spectroscopy might form the basis for quantitation of glucose and urea levels in whole blood. Spectra were acquired by first diluting whole blood specimens with a dilute potassium thiocyanate solution, drying small aliquots to dry films on BaF2 substrates and acquiring transmission spectra for the dry films. Reference analyses for urea and glucose were carried out, and partial least-squares calibrations developed using two-thirds of the 200 total specimens for calibration and the remaining one-third to validate the analytical methods. A potassium thiocyanate absorption at 2060 cm−1 provided the means to normalize the spectra, factoring out any minor variations in film thickness.
Publication date
AffiliationNRC Institute for Biodiagnostics; NRC Genomics and Health Initiative; National Research Council Canada
Peer reviewedYes
NRC number1907
NPARC number9148546
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Record identifieref0ee926-bc18-47a3-8193-97d4e223ae13
Record created2009-06-25
Record modified2016-09-29
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