Spontaneous formation of small unilamellar vesicles by pH jump : a pH gradient across the bilayer membrane as the driving force

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DOIResolve DOI: http://doi.org/10.1021/bi00461a016
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TypeArticle
Journal titleBiochemistry
ISSN0006-2960
1520-4995
Volume29
Issue9
Pages23212329
Abstract³¹P NMR and infrared spectroscopic methods have been used to study the formation of small unilamellar vesicles by the pH-jump method. It is shown that increasing the pH of different lamellar phospholipid dispersions (phosphatidic acids and phosphatidylserines) induces a pH gradient. This pH gradient is estimated to be 4 ± 1 pH units, and its direction is such that the inner monolayer of the vesicles is at lower pH. There is spectroscopic evidence for tighter packing of the lipid hydrocarbon chains in the inner monolayer, probably due to the constraints imposed by the high curavture of the small vesicles formed. There results are discussed in terms of the driving force of the spontaneous vesiculation.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NRC number30762
NPARC number23001506
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Record identifierefc4bae8-f526-4069-a800-3ce19453f4c1
Record created2017-02-20
Record modified2017-02-20
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