Multi-laboratory study of five methods for the determination of brevetoxins in shellfish tissue extracts

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TypeArticle
Proceedings titleProceedings of the 10th International Conference on Harmful Algae, St. Pete Beach, Florida, Oct. 21-25, 2002
Conference10th International Conference on Harmful Algae, Oct. 21-25, 2002, St. Pete Beach, Florida, USA
Pages300302
AbstractA thirteen-laboratory comparative study tested the performance of four methods as alternatives to mouse bioassay for the determination of brevetoxins in shellfish. The methods were N2a neuroblastoma cell assay, two variations of the sodium channel receptor binding assay, competitive ELISA, and LC/MS. Three to five laboratories independently performed each method using centrally prepared spiked and naturally incurred test samples. Competitive ELISA and receptor binding (96-well format) compared most favorably with mouse bioassay. Between-laboratory relative standard deviations (RSDR) ranged from 10 to 20% for ELISA and 14 to 31% for receptor binding. Within-laboratory (RSDr) ranged from 6 to 15% for ELISA, and 5 to 31% for receptor binding. Cell assay was extremely sensitive but data variation rendered it unsuitable for statistical treatment. LC/MS performed as well as ELISA on spiked test samples but was inordinately affected by lack of toxin-metabolite standards, uniform instrumental parameters, or both, on incurred test samples. The ELISA and receptor binding assay are good alternatives to mouse bioassay for the determination of brevetoxins in shellfish.
Publication date
PublisherFlorida Fish and Wildlife Conservation Commission, Florida Institute of Oceanography, and Intergovernmental Oceanographic Commission of UNESCO
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NPARC number23001102
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Record identifierf03cb109-1d9f-47d1-81c1-a1b765611ac1
Record created2016-12-13
Record modified2016-12-13
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