A mussel tissue certified reference material for multiple phycotoxins. Part 3: homogeneity and stability

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DOIResolve DOI: http://doi.org/10.1007/s00216-011-4787-8
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TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
Volume400
Issue3
Pages847858; # of pages: 12
SubjectCRM-FDMT1; Homogeneity; HPLC; LC-MS; Phycotoxins; Shellfish toxins; Stability
AbstractA candidate certified reference material (CRM) for multiple shellfish toxins (domoic acid, okadaic acid and dinophysistoxins, pectenotoxins, yessotoxin, azaspiracids and spirolides) has been prepared as a freeze-dried powder from mussel tissues (Mytilus edulis). Along with the certified values, the most important characteristics for a reference material to be fit-for-purpose are homogeneity and stability. Acceptable between-bottle homogeneity was found for this CRM. Within-bottle homogeneity was assessed using domoic acid, and it was shown that repeated subsampling of the CRM can be performed precisely down to 0.35 g. Both short- and long-term stability studies carried out under isochronous conditions demonstrated excellent stability of the various toxins present in the material. While degradation of some analytes was observed at +60°C in short-term studies, it was determined that shipping at ambient temperature is adequate. No instability was detected in long-term stability studies, and it was shown that the material can be held at +18°C safely for up to 1 year. To guarantee stability of the CRM over its lifetime the stock will be maintained at -20°C. The results of the homogeneity and stability testing show that CRM-FDMT1 is appropriate for its intended use in quality assurance and quality control of shellfish toxin analysis methods.
Publication date
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NPARC number19709150
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Record identifierf232de2f-c8e1-4783-9c57-d3c6677ecc19
Record created2012-04-02
Record modified2016-05-09
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