Development, characterization, and functional activity of a panel of specific monoclonal antibodies to inner core lipopolysaccharide epitopes in Neisseria meningitidis: Infect.Immun.

Download
  1. Get@NRC: Development, characterization, and functional activity of a panel of specific monoclonal antibodies to inner core lipopolysaccharide epitopes in Neisseria meningitidis: Infect.Immun. (Opens in a new window)
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleAcoustics, Speech, and Signal Processing Newsletter, IEEE
Volume72
Issue1
Pages559569; # of pages: 11
SubjectAntibodies; antibody; assay; bacterial; binding; Blood; Canada; cell; CELL-SURFACE; DISEASE; Enzyme Linked Immunosorbent Assay; Enzyme-Linked Immunosorbent Assay; epitope; Epitopes; FLUID; Infant; LIPOPOLYSACCHARIDE; LPS; MONOCLONAL-ANTIBODIES; MONOCLONAL-ANTIBODY; Neisseria; Neisseria meningitidis; PROTECTION; SAMPLES; STRAIN; STRAINS; surface; Vaccines
AbstractA panel of six murine monoclonal antibodies (MAbs) recognizing inner core lipopolysaccharide (LPS) epitopes of Neisseria meningitidis was prepared and characterized in order to determine the diversity of inner core LPS glycoforms among disease and carrier isolates. Two of these MAbs, L2-16 (immunoglobulin G2b [IgG2b]) and LPT3-1 (IgG2a), together with a third, previously described MAb, L3B5 (IgG3), showed reactivity, either individually or in combination, with all except 3 of 143 disease and carriage isolates (125 of 126 strains from blood, cerebrospinal fluid, or skin biopsy samples and 15 of 17 from nasopharyngeal cultures). MAbs L3B5, L2-16, and LPT3-1 were further characterized in an indirect immunofluorescence assay. All three MAbs bound to the bacterial cell surface, findings that correlated strongly with whole-cell enzyme-linked immunosorbent assay and immunodot blots. However, in contrast to our findings with L3B5, cell surface binding of L2-16 or LPT 3-1 did not correlate with functional activity as determined by bactericidal or infant rat passive protection assays against wild-type N. meningitidis strains. These findings are provocative with respect to the requirements for protective activity of antibodies and the development of inner core LPS vaccines against invasive meningococcal disease
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberGIDNEY2004
NPARC number9360960
Export citationExport as RIS
Report a correctionReport a correction
Record identifierf28417eb-bb18-4882-ab43-e573cab233f0
Record created2009-07-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)