Helicobacter pylori isolates from Greek children express type 2 and type 1 Lewis and α1, 6-glucan antigens in conjunction with a functional type IV secretion system

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DOIResolve DOI: http://doi.org/10.1099/jmm.0.038729-0
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TypeArticle
Journal titleJournal of Medical Microbiology
ISSN0022-2615
Volume61
Issue4
Pages559566; # of pages: 8
Subjectalpha1,6 glucan antigen; CagA protein; epitope; glucan; unclassified drug; adolescent; article; bacterial strain; bacterium isolate; child; enzyme linked immunosorbent assay; gel electrophoresis; Greece; Helicobacter infection; Helicobacter pylori; human; human tissue; immunoblotting; nonhuman; pathogenicity; pediatrics; preschool child; priority journal; school child; Adolescent; Antigens, Bacterial; Bacterial Proteins; Child; Child, Preschool; Gastric Mucosa; Gene Expression Regulation, Bacterial; Glucans; Greece; Helicobacter Infections; Helicobacter pylori; Humans; Lewis Blood-Group System
AbstractHelicobacter pylori infection is often acquired in childhood and can persist for life. Previous studies in adult patients have shown that H. pylori isolates from North American and European hosts express predominantly type 2 Lewis x (Le x) and Le y epitopes, while Asian strains have the capacity to express type 1 Le a and Le b structures. In order to understand the influence of environmental and host factors on the expression of Le antigens, we analysed 50 Greek H. pylori isolates from symptomatic children. Both CagA-positive and-negative strains were evaluated. The expression of Le antigens was determined by whole-cell indirect ELISA (WCE), and LPS profiles were assessed by gel electrophoresis and immunoblotting. Occurrence of Le x and/or Ley antigens was confirmed in 35 of the isolates (70 %) while 15 of the isolates were non-typable. It was found that 11 of the paediatric isolates had the propensity to express type 1 Le b blood-group antigen (22 %), a feature relatively uncommon in H. pylori isolates from adults. One strain expressed both Le b and Le a antigens. The majority of the isolates (49/50, 98 %) expressed α1,6-glucan, an antigenic non-Le determinant present in the outer core region of H. pylori LPS. All Le x-and Leyexpressing strains also carried a functional cag pathogenicity island-encoding a type IV secretion system, capable of translocating CagA protein, as well as the vacAs1 allele, suggesting that Le x and Ley epitopes may aid the persistence of more aggressive strains. No association between bacterial virulence characteristics and the histopathological observations was evident. © 2012 National Research Council of Canada.
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LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269304
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Record identifierf2cf38b0-cf70-4dd1-a788-a78e04a9efa9
Record created2013-12-12
Record modified2016-05-09
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