Specific inhibitors for identifying pathways for methane production from carbon monoxide by a nonadapted anaerobic mixed culture

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DOIResolve DOI: http://doi.org/10.1139/cjm-2013-0843
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TypeArticle
Journal titleCanadian Journal of Microbiology
Volume60
Issue6
Pages407415; # of pages: 9
Subjectcarbon monoxide, anaerobic, methanation, methanogenesis, carboxydotrophy, bromoethanesulfonate (BES), vancomycin
AbstractSpecific inhibitors such as 2-bromoethanesulfonate (BES) and vancomycin were employed in activity batch tests to decipher metabolic pathways that are preferentially used by a mixed anaerobic consortium (sludge from an anaerobic digester) to transform carbon monoxide (CO) into methane (CH₄). We first evaluated the inhibitory effect of both BES and vancomycin on the microbial community, as well as the efficiency and stability of vancomycin at 35 °C, over time. The activity tests with CO₂–H₂, CO, glucose, acetate, formate, propionate, butyrate, methanol, and ethanol showed that vancomycin does not inhibit some Gram-negative bacteria, and 50 mmol/L BES effectively blocks CH₄ production in the sludge. However, when sludge was incubated with propionate, butyrate, methanol, or ethanol as the sole energy and carbon source, methanogenesis was only partially inhibited by BES. Separate tests showed that 0.07 mmol/L vancomycin is enough to maintain its inhibitory efficiency and stability in the population for at least 32 days at 35 °C. Using the inhibitors above, it was demonstrated that CO conversion to CH₄ is an indirect, 2-step process, in which the CO is converted first to acetate and subsequently to CH₄.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; Energy, Mining and Environment
Peer reviewedYes
NRC numberNRC-EME-55643
NPARC number21272073
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Record identifierf3bbdaae-71bc-4d8d-ba0b-4b9f3c7db3d9
Record created2014-06-18
Record modified2016-05-09
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