Near-Field Scanning Optical Microscopy to Identify Membrane Microdomains

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TypeBook Chapter
Book titleMethods in Membrane Lipids
Pages469480; # of pages: 12
AbstractNear-field scanning optical microscopy (NSOM) allows optical imaging with a spatial resolution that is significantly better than the diffraction limited resolution achievable with conventional optical microscopy. NSOM has the potential to study the nanoscale organization of membrane surfaces and ultimately to resolve questions concerning lipid rafts in both model-supported membranes and cellular membranes. Supported phospholipid monolayers and bilayers of phase separated binary and ternary lipid mixtures that model the composition of lipid rafts in natural membranes have been studied by NSOM. The results illustrate the ability of NSOM measurements with 50�100 nm probe apertures to obtain detailed nanoscale information for small, closely spaced domains, and the utility of two-color experiments to probe localization of raft markers in supported membranes.
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedNo
NPARC number12339332
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Record identifierf527ada2-aa3a-405f-9a56-30ba1f5fe6b3
Record created2009-09-11
Record modified2016-05-09
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