Multianalyte serum analysis using mid-infrared spectroscopy

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Journal titleAnnals of Clinical Biochemistry
Pages624632; # of pages: 9
Subjectclinical chemistry analyses; urea; glucose; protein; albumin
AbstractThis study assesses the potential for using mid-infrared (mid-IR) spectroscopy of dried serum films as the basis for the simultaneous quantitation of eight serum analytes: Total protein, albumin, triglycerides, cholesterol, glucose, urea, creatinine and uric acid. Infrared transmission spectra were acquired for 300 serum samples, each analysed independently using accepted reference clinical chemical methods. Quantitation methods were based upon the infrared spectra and reference analyses for 200 specimens, and the models validated using the remaining 100 samples. Standard errors in the IR-predicted analyte levels (Sy/x) were 2.8 g/L (total protein), 2.2 g/L (albumin), 0.23 mmol/L (triglycerides), 0.28 mmol/L (cholesterol), 0.41 mmol/L (glucose) and 1.1 mmol/L for urea, with correlation coefficients (IR vs reference analyses) of 0.95 or better. The IR method emerged to be less suited for creatinine (Sy/x = μmol/L) and uric acid (Sy/x = 140 μmol/L) due to the relatively low concentrations typical of these analytes.
Publication date
PublisherAssociation for Clinical Biochemistry and Laboratory Medicine
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NRC number883
NPARC number9148283
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Record identifierf619a7e2-0c77-4d17-ace3-24f0dc2106b9
Record created2009-06-25
Record modified2016-10-14
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