Transcriptional and metabolomic analysis of Ascophyllum nodosum mediated freezing tolerance in Arabidopsis thaliana

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Journal titleBMC Genomics
Article number643
Subjectcarboxylic acid; fatty acid; proline; proline dehydrogenase; pyrroline 5 carboxylate reductase; sugar; sugar alcohol; Arabidopsis; article; Ascophyllum; ascophyllum nodosum; controlled study; cytosol; down regulation; freezing; freezing tolerance; gene expression; genetic transcription; heteronuclear multiple bond correlation; heteronuclear single quantum coherence; lipid composition; lipophilicity; metabolomics; nonhuman; nuclear magnetic resonance spectroscopy; osmotic stress; P5CS1 gene; P5CS2 gene; plant gene; ProDH gene; proton nuclear magnetic resonance; sfr4 gene; transcriptomics; upregulation; Arabidopsis; Ascophyllum; Carbohydrate Metabolism; Freezing; Gene Expression Profiling; Hot Temperature; Hydrophobic and Hydrophilic Interactions; Metabolomics; Mutation; Proline; Solubility; Transcription, Genetic; Arabidopsis; Arabidopsis thaliana; Ascophyllum nodosum
AbstractBackground: We have previously shown that lipophilic components (LPC) of the brown seaweed Ascophyllum nodosum (ANE) improved freezing tolerance in Arabidopsis thaliana. However, the mechanism(s) of this induced freezing stress tolerance is largely unknown. Here, we investigated LPC induced changes in the transcriptome and metabolome of A. thaliana undergoing freezing stress.Results: Gene expression studies revealed that the accumulation of proline was mediated by an increase in the expression of the proline synthesis genes P5CS1 and P5CS2 and a marginal reduction in the expression of the proline dehydrogenase (ProDH) gene. Moreover, LPC application significantly increased the concentration of total soluble sugars in the cytosol in response to freezing stress. Arabidopsis sfr4 mutant plants, defective in the accumulation of free sugars, treated with LPC, exhibited freezing sensitivity similar to that of untreated controls. The 1H NMR metabolite profile of LPC-treated Arabidopsis plants exposed to freezing stress revealed a spectrum dominated by chemical shifts (δ) representing soluble sugars, sugar alcohols, organic acids and lipophilic components like fatty acids, as compared to control plants. Additionally, 2D NMR spectra suggested an increase in the degree of unsaturation of fatty acids in LPC treated plants under freezing stress. These results were supported by global transcriptome analysis. Transcriptome analysis revealed that LPC treatment altered the expression of 1113 genes (5%) in comparison with untreated plants. A total of 463 genes (2%) were up regulated while 650 genes (3%) were down regulated.Conclusion: Taken together, the results of the experiments presented in this paper provide evidence to support LPC mediated freezing tolerance enhancement through a combination of the priming of plants for the increased accumulation of osmoprotectants and alteration of cellular fatty acid composition. © 2012 Nair et al.; licensee BioMed Central Ltd.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Nutrisciences and Health (INH-ISNS)
Peer reviewedYes
NPARC number21269265
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Record identifierf6a4f123-afcf-4484-8d05-ecbfc52c38c4
Record created2013-12-12
Record modified2016-05-09
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