Structure of the antigenic repeating pentasaccharide unit of the LPS O-polysaccharide of Cronobacter sakazakii implicated in the Tennessee outbreak

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DOIResolve DOI: http://doi.org/10.1139/O09-004
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TypeArticle
Journal titleBiochemistry and Cell Biology
Volume87
Issue2
Pages459465; # of pages: 7
SubjectO-polysaccharide; Cronobacter sakazakii; NMR; LPS; O-antigen; Cronobacter sakazakii; RMN; LPS; antigène O
AbstractStrains of the Gram-negative bacterium Cronobacter (Enterobacter) sakazakii have been identified as emerging opportunistic pathogens that can cause enterocolitis, bacteraemia, meningitis, and brain abscess, and have been particularly associated with meningitis in neonates where infant-milk formulae has been epidemiologically linked to the disease. A study of the lipopolysaccharides produced by clinical isolates using chemical, 2D ¹H and ¹³C NMR, and MS methods revealed that the O-polysaccharide produced by C. sakazakii (3290), a clinical strain from the Tennessee outbreak, was a branched polymer of repeating pentasaccharide units composed of 2-acetamido-2-deoxy-D-galactose, 3-(N-acetyl-L-alanylamido)-3-deoxy-D-quinovose, D-glucuronic acid, and D-glucose present in the molar ratio 1:1:1:2. The O-PS structure provides a unique specific structurally defined marker for the clinical tracking of this pathogen.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences
Peer reviewedYes
NPARC number15336761
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Record identifierf75f3398-ad7d-4dd6-a121-f342407df9a0
Record created2010-05-21
Record modified2016-05-09
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