A scalable process for helper-dependent adenoviral vector production using pei-derived transfection strategy in suspension culture

DOIResolve DOI: http://doi.org/10.1007/978-90-481-3419-9_77
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Proceedings titleCells and Culture: Proceedings of the 20th ESACT Meeting, Dresden, Germany, June 17-20, 2007
Series titleESACT Proceedings; no. Vol. 4
Conference20th ESACT Meeting, June 17-20, 2007, Dresden, Germany
Subjecthelper-dependent adenoviral vector; HEK 293; PEI-transfection; suspension cells; bioreactor
AbstractHelper-dependent adenoviral vector has demonstrated a high therapeutic potential; however, the poor efficiency of the actual production process hampers further large-scale clinical evaluation. In this work, we evaluated the adenofection protocol, an efficient polyethylenimine transfection strategy, for HDV production. Optimized conditions of PEI-adenofection were used to generate HDV at 3-L bioreactor scale. This process strategy realized in suspension reduced process duration and therefore ensured production of high-quality vector stock.
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AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NPARC number23001980
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Record identifierf7673588-623f-4a5b-8cd5-0cedb223d1d6
Record created2017-07-12
Record modified2017-07-12
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