Conserved glycolipid termini in capsular polysaccharides synthesized by ATP-binding cassette transporterdependent pathways in Gram-negative pathogens

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DOIResolve DOI: http://doi.org/10.1073/pnas.1222317110
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TypeArticle
Journal titleProceedings of the National Academy of Sciences of the United States of America
ISSN0027-8424
Volume110
Issue19
Pages78687873; # of pages: 6
SubjectABC transporter; glycerophospholipid; glycolipid; glycosaminoglycan polysulfate; polysaccharide; polysialic acid; amino acid sequence; article; bacterial capsule; biosynthesis; Escherichia coli; hydrophobicity; lipid analysis; molecular weight; Neisseria meningitidis; nonhuman; priority journal; proton nuclear magnetic resonance; capsule assembly; polysaccharide export; Adenosine Triphosphate; ATP-Binding Cassette Transporters; Bacterial Capsules; Biological Transport; Escherichia coli; Glycolipids; Gram-Negative Bacteria; Magnetic Resonance Spectroscopy; Mass Spectrometry; Methylation; Mutation; Neisseria meningitidis; Polysaccharides; Virulence Factors
AbstractBacterial capsules are surface layers made of long-chain polysaccharides. They are anchored to the outer membrane of many Gramnegative bacteria, including pathogens such as Escherichia coli, Neisseriameningitidis, Haemophilus influenzae, andPasteurella multocida. Capsules protect pathogens from host defenses including complement- mediated killing and phagocytosis and therefore represent a major virulence factor. Capsular polysaccharides are synthesized by enzymes located in the inner (cytoplasmic) membrane and are then translocated to the cell surface.Whereas the enzymes that synthesize the polysaccharides have been studied in detail, the structure and biosynthesis of the anchoring elements have not been definitively resolved. Here we determine the structure of the glycolipid attached to the reducing terminus of the polysialic acid capsular polysaccharides from E. coli K1 and N. meningitidis group B and the heparosan-like capsular polysaccharide from E. coli K5. All possess the same unique glycolipid terminus consisting of a lyso-phosphatidylglycerol moiety with a β-linked poly-(3-deoxy-D-manno-oct-2-ulosonic acid) (poly-Kdo) linker attached to the reducing terminus of the capsular polysaccharide.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270615
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Record identifierf820ac5a-63c5-40ff-8c30-857e9f9cbb2e
Record created2014-02-17
Record modified2016-05-09
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