Quantitative analysis of azaspiracids in Azadinium spinosum cultures

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DOIResolve DOI: http://doi.org/10.1007/s00216-012-5849-2
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
ISSN1618-2642
1618-2650
Volume403
Issue3
Pages833846; # of pages: 14
Subjectextraction procedure; extraction artefact; matrix effects; LC-MS/MS; azaspiracid methyl ester; dinoflagellate; liquid chromatography–mass spectrometry
AbstractAzaspiracids (AZAs) are secondary metabolites of Azadinium spinosum that can accumulate in shellfish and cause food poisoning when consumed. We describe here an analytical procedure for the determination of AZAs in cultures of A. spinosum with a focus on the formation of AZA methyl esters as artefacts during extraction and sample pre-treatment. A. spinosum cells were collected from bioreactor cultures using centrifugation or filtration. Different extraction procedures were evaluated for formation of methyl ester artefacts, yield, and matrix effects. Filtration of cultures using glass-fibre filters led to increased formation of methyl esters, and centrifugation is recommended for recovery of cells. The extraction solvent (methanol (MeOH), acetone, and acetonitrile (MeCN)) did not significantly affect the yield of AZAs as long as the organic content was 80% or higher. However, the use of MeOH as extraction solvent led to increased formation of methyl esters. AZA1 recovery over two successive extractions was 100% at the 95% confidence level for acetone and MeOH. In standard-addition experiments, no significant matrix effects were observed in extracts of A. spinosum or Azadinium obesum up to a sample size of 4.5 × 10^9 μm^3. Moreover, experiments carried out to clarify the formation and structure of methylated AZA analogues led to the description of two AZA methyl esters and to the correction of the chemical structures of AZAs29–32.
Publication date
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
Identifier5849
NPARC number21268285
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Record identifierf8da29fd-9997-4969-ba07-f2a29686080c
Record created2013-06-13
Record modified2016-05-09
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