Determination of antibody affinity by surface plasmon resonance

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TypeBook Chapter
Book titleAnticarbohydrate Antibodies: From Molecular Basis to Clinical Application
Pages403429; # of pages: 27
AbstractOver the past two decades surface plasmon resonance (SPR) has emerged as gold standard technology for the analysis of biomolecular interactions. In the monoclonal antibody (mAb) development area SPR is employed from the early screening stages to detailed characterization of binding kinetics and affinities to epitope mapping to final product testing. This label free technology provides high quality kinetic and affinity data and, in some instances, other unique information not obtained by alternative methods such as ELISA. SPR is well-suited to the study of protein-carbohydrate interactions which are often of low affinity and not easily characterized by other methods.
Publication date
PublisherSpringer International Publishing
AffiliationNational Research Council Canada (NRC-CNRC); Human Health Therapeutics
Peer reviewedYes
NPARC number21275592
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Record identifierf8fc3930-a093-4628-a8b9-5d649471ac64
Record created2015-07-14
Record modified2016-05-09
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