The first description of a (1--> 6)-beta-D-glucan in prokaryotes: (1 --> 6)-beta-D-glucan is a common component of Actinobacillus suis and is the basis for a serotyping system: Carbohydr.Res.

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TypeArticle
Journal titleCarbohydr.Res.
Volume329
Issue1
Pages121130; # of pages: 10
SubjectActinobacillus; Actinobacillus suis; Algorithms; Animals; Antibodies; antibody; bacterial; Canada; capsular polysaccharide; capsular polysaccharides; Carbohydrate Conformation; Carbohydrate Sequence; cell; Cell Wall; CELL-SURFACE; chemical; chemistry; COMPONENT; COMPONENTS; CONFORMATIONAL; CPS; Cross Reactions; GLUCANS; Immune Sera; Immunoblotting; immunology; INFECTION; K1; linear; LIPOPOLYSACCHARIDE; Lipopolysaccharides; MAGNETIC; MAGNETIC-RESONANCE; Mass Fragmentography; metabolism; Microbiology; Models,Molecular; Molecular Sequence Data; NUCLEAR; Nuclear Magnetic Resonance; Nuclear Magnetic Resonance,Biomolecular; NUCLEAR-MAGNETIC-RESONANCE; O-chain; O1; PIGS; POLYSACCHARIDE; Polysaccharides; Polysaccharides,Bacterial; RESONANCE; SEROTYPE; Serotyping; serotyping system; STRAIN; STRAINS; structure; Support,Non-U.S.Gov't; Swine; SYSTEM; Thermodynamics
AbstractThe chemical and antigenic properties of the cell-surface lipopolysaccharides (LPSs) and capsular polysaccharides (CPSs) of seven representative strains of Actinobacillus suis from healthy and diseased pigs were investigated. Four strains produced a linear (1 --> 6)-beta-D-glucan homopolymer, beta-D-Glcp-(1-[ --> 6)-beta-D-Glcp-(1-]n -->, as a LPS-O-chain (O1) and as a CPS (K1). Polyclonal antisera prepared against a (1 --> 6)-beta-D-glucan-containing strain showed a positive reaction against both LPSs and CPSs derived from the above strains (designated serotype O1/K1). One strain carried the (1 --> 6)-beta-D-glucan solely as a LPS-O-chain (serotype O1) and two strains did not express the (1 --> 6)-beta-D-glucan, but, instead, produced a different O-chain (designated serotype 02); these three strains expressed their own characteristic CPSs. (1 --> 6)-beta-D-Glucan structures are common cell wall components of yeast, fungi and lichens, but, to our knowledge, this is the first time a (1 --> 6)-beta-D-glucan has been described in a prokaryotic organism. Conformational and nuclear magnetic resonance analyses showed that the beta-D-Glcp-(1 --> 6)-beta-D-Glcp linkage was flexible and two distinct glycosidic conformers are described. Cross-reactive antibodies to the A. suis (1 --> 6)-beta-D-glucan could be detected in sera from a variety of species and in sera from specific pathogen free pigs. This cross-reactivity may arise from immuno-stimulation of organisms present in the surrounding environment that contain (1 --> 6)-beta-D-glucan, which may also explain the high incidence of false positive results in previous serological tests for A. suis. In addition, these (1 --> 6)-beta-D-glucan background antibodies may be protective against A. suis infection. The characterization herein of (1 --> 6)-beta-D-glucan is the foundation for the development of a serotyping system for A. suis
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberMONTEIRO2000B
NPARC number9358470
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Record identifierf95718f8-3c03-40f0-9fce-1d84e71e8878
Record created2009-07-10
Record modified2016-05-09
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