Isolation of funcitonal single domain antibody by whole cell immunization: Implications for cancer treatment.

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DOIResolve DOI: http://doi.org/10.1016/j.jim.2011.06.017
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TypeArticle
Journal titleJournal of Immunlogical Methods
Volume371
Issue1-2
Pages7080; # of pages: 11
SubjectSingle domain antibody (sdAb); CEACAM6; Pancreatic cancer; Immunotherapy
AbstractCarcinoembryonic antigen related cell adhesion molecule (CEACAM) 6 is over-expressed in different types of cancer cells. In addition, it has also been implicated in some infectious diseases. Targeting this molecule by an antibody might have applications in diverse tumor models. Single domain antibody (sdAb) is becoming very useful format in antibody engineering as potential tools for treating acute and chronic disease conditions such as cancer for both diagnostic as well as therapeutic application. Generally, sdAbs with good affinity are isolated from an immune library. Discovery of a new target antigen would require a new immunization with purified antigen which is not always easy. In this study, we have isolated, by phage display, an sdAb against CEACAM6 with an affinity of 5 nM from a llama immunized with cancer cells. The antibody has good biophysical properties, and it binds to the cells expressing the target antigen. Furthermore, it reduces cancer cells proliferation in vitro and shows an excellent tumor targeting in vivo. This sdAb could be useful in diagnosis as well as therapy of CEACAM6 expressing tumors. Finally, we envisage it would be feasible to isolate good sdAbs against other interesting tumor associated antigens from this library. Therefore, this immunization method could be a general strategy for isolating sdAbs against any surface antigen without immunizing the animal with the antigen of interest each time.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number19098657
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Record identifierf9e442cf-5894-4d1d-8546-29a2b7cda3f1
Record created2011-12-20
Record modified2016-05-09
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