The efficacy of new colchicine derivatives and viability of the T-Lymphoblastoid cells in three-dimensional culture using 19F MRI and HPLC-UV ex vivo

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DOIResolve DOI: http://doi.org/10.1016/j.bioorg.2009.07.007
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TypeArticle
Journal titleBioorganic Chemistry
Volume37
Issue6
Pages193201; # of pages: 9
Subjectbioreactors; cell culture techniques; cell survival; chromatography
AbstractThe paper describes ex vivo applications of colchicine derivatives for the treatment of human T-Lymphoblastoid (CEM) cells. Moreover, the role of the substitutions of ring A at C-1 and C-7 side chain of colchicine analogues was probed by the synthesis and examination of their effects on the three-dimensional (3-D) CEM cells' growth. The CEM cells were cultured in the hollow fiber bioreactor (HFB) device. We used (1)H and (19)F magnetic resonance imaging (MRI) to monitor changes in 3-D CEM cell culture. (19)F MRI was used for visualization of the cellular uptake of new fluorine derivatives. Before and after treatment CEM cells profile was investigated with high performance liquid chromatography (HPLC-UV).
Publication date
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; Medical Devices; National Research Council Canada
Peer reviewedYes
NPARC number20001145
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Record identifierfa91ef9a-94ea-480c-9b9f-31736dd48a4a
Record created2012-05-25
Record modified2016-05-09
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