Identification and structural characterization of a sialylated lacto-N-neotetraose structure in the lipopolysaccharide of Haemophilus influenzae

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TypeArticle
Journal titleEuropean Journal of Biochemistry
Volume269
Issue16
Pages40094019; # of pages: 11
SubjectACID; Canada; COMPONENT; CORE REGION; genetics; Haemophilus; Haemophilus influenzae; HAEMOPHILUS-INFLUENZAE; Human; IDENTIFICATION; INNER CORE REGION; LIPOPOLYSACCHARIDE; LPS; MOLECULAR; MOLECULE; MS; NMR; NMR spectroscopy; NMR-spectroscopy; oligosaccharide; REGION; RESIDUES; SIALIC; SIALIC-ACID; SPECTROSCOPY; STRAIN; STRUCTURAL; STRUCTURAL CHARACTERIZATION; structure; technique; UNIT
AbstractA sialylated lacto-N-neotetraose (Sial-lNnT) structural unit was identified and structurally characterized in the lipopolysaccharide (LPS) from the genome-sequenced strain Road (RM118) of the human pathogen Haemophilus influenzae grown in the presence of sialic acid. A combination of molecular genetics, MS and NMR spectroscopy techniques showed that this structural unit extended from the proximal heptose residue of the inner core region of the LPS molecule. The structure of the Sial-lNnT unit was identical to that found in meningococcal LPS, but glycoforms containing truncations of the Sial-lNnT unit, comprising fewer residues than the complete oligosaccharide component, were not detected. The finding of sialylated glycoforms that were either fully extended or absent suggests a novel biosynthetic feature for adding the terminal tetrasaccharide unit of the Sial-lNnT to the glycose acceptor at the proximal inner core heptose
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NRC numberCOX2002
NPARC number9372137
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Record identifierfa9d7061-fb18-4222-8fec-ff740adfd07f
Record created2009-07-10
Record modified2016-06-01
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