In vitro assay for ABA 8'-hydroxylase: implications for improved assays for cytochrome P450 enzymes

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DOIResolve DOI: http://doi.org/10.1007/978-1-61779-231-1_8
AuthorSearch for: ; Search for:
TypeArticle
Journal titleSeed Dormancy: Methods and Protocols
Series titleMethods in Molecular Biology; Volume 733
ISBN978-1-61779-230-4
Pages113134; # of pages: 22
Subjectcytochrome P450; in vitro assay; microsomes; (+) -ABA 8'-hydroxylase; abscisic acid; dormancy; germination; phaseic acid
AbstractIn vitro assays for cytochrome P450 enzymes developed from plant-derived microsomal extracts have not been used extensively for the characterization and quantification of enzyme activities in plant tissues. We describe here an in vitro assay for abscisic acid (ABA) 8 -hydroxylase that was developed using microsomes extracted from ( )-ABA-induced corn suspension cultures. This assay may be useful for further characterization and monitoring of ABA 8 -hydroxylase activities in germinating seeds, seedlings, and other tissues. Additionally, the optimization protocols provided here may be adapted towards improving in vitro enzyme assays for other cytochrome P450 enzymes expressed in plants.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NRC number50161
NPARC number18906336
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Record identifierfac896bf-c28c-4104-8b1e-365818d74faf
Record created2012-04-01
Record modified2016-05-09
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