A biosensor system for chlorophenols using chloroperoxidase and a glucose oxidase based amperometric electrode

Download
  1. Get@NRC: A biosensor system for chlorophenols using chloroperoxidase and a glucose oxidase based amperometric electrode (Opens in a new window)
AuthorSearch for: ; Search for:
TypeArticle
Journal titleElectroanalysis
Volume10
Issue1
Pages711; # of pages: 5
SubjectChlorophenols; Biosensor; Glucose oxidase; Chloroperoxidase
AbstractA combined electrocatalytic approach using chloroperoxidase and a glucose oxidase electrode has been developed for the measurement of chlorophenol (CP) congeners. Chloroperoxidase possessed a capability to generate a significant or detectable amount of electroactive species from the oxidation of 4-CP, 2,3-dichlorophenol (DCP), 2,4-DCP, 2,5-DCP, 3,4-DCP, 3,5-DCP, 2,3,4-trichlorophenol (TCP), 2,3,5-TCP, 2,3,6-TCP, 2,4,5-TCP, 2,4,6-TCP, and 2,3,4,6-tetraCP. The optimal oxidation conditions were established as 33 μM chlorophenol, 33 U/mL chloroperoxidase and 1.33 mM hydrogen peroxide for 15 min at ambient temperature. The chlorophenol oxidation products have also been characterized using UV-vis spectrophotometry and cyclic voltammetry (CV). Together with glucose oxidase immobilized on a working glassy carbon electrode (+0.45 V vs. Ag/AgCl), chlorinated oxidation products have been demonstrated as efficient mediators in a glucose oxidase/glucose system. However, the oxidation products from the enzymatic reaction with 2-CP, 3-CP, 2,6-DCP, 3,4,5-TCP, 2,3,5,6-tetraCP and pentachlorophenol were not electroactive or capable of recycling reduced glucose oxidase to its reactive state.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number40534
NPARC number3539318
Export citationExport as RIS
Report a correctionReport a correction
Record identifierfb09c023-8dcd-4ceb-bbd7-e2580ba84250
Record created2009-03-01
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)