Conformational changes in the cyclic undecapeptide cyclosporin induced by interaction with metal ions. An FTIR study

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DOIResolve DOI: http://doi.org/10.1016/0141-8130(94)90041-8
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TypeArticle
Journal titleInternational Journal of Biological Macromolecules
ISSN0141-8130
Volume16
Issue3
Pages143148; # of pages: 6
Subjectcyclosporin; infra-red spectroscopy; metal ions
AbstractInfra-red spectra have been measured for the cyclic undecapeptide cyclosporin A (CsA) and three analogues CsC, CsD and CsH in acetonitrile and in the presence of 10:1 molar excess of Mg²⁺, Ca²⁺, Na⁺ or Li⁺ in the same solvent. Interaction with each of these ions is suggested by marked changes in band positions over the amide 1 region (1600–1700 cm⁻¹). The formation of complexes of cyclosporin with calcium and magnesium ions is indicated by the presence of C=O stretching bands well outside the range normally expected for the amide I absorptions of free peptides. Although they share this characteristic, the spectra indicate that the mode and/or strength of Ca²⁺ binding is quite different from that of Mg²⁺ binding. In contrast, the two monovalent ions interact with CsA, CsC and CsD to yield spectra that are very similar to one another. The spectra are consistent with binding of the monovalent ions simultaneously to several carbonyl groups of the loop structure.
Publication date
PublisherButterworth-Heinemann Limited
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biodiagnostics
Peer reviewedYes
NRC number295
NPARC number9722514
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Record identifierfb63975f-8dcb-42b1-91ae-1e8169d8c1d3
Record created2009-07-17
Record modified2016-12-06
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