MALDI mass spectrometry imaging of gangliosides in mouse brain using ionic liquid matrix

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DOIResolve DOI: http://doi.org/10.1016/j.aca.2009.02.051
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TypeArticle
Journal titleAnalytica Chimica Acta
Volume639
Issue1-2
Pages5761; # of pages: 5
SubjectMass spectrometry imaging; Ganglioside; Matrix-assisted laser desorption/ionization; time-of-flight; Mouse brain; Ceramide; Hippocampus
AbstractMass spectrometry imaging has emerged as a powerful tool for the direct detection of biomolecules, mainly phospholipids, proteins and peptides, in tissue samples. To date, there is very little information available on the direct analysis of gangliosides in brain tissue. One major hurdle for imaging gangliosides in tissue using mass spectrometry is that sialic acid residues can be dissociated in ionization process. In this report, we investigated an ionic liquid matrix for mass spectrometry imaging of gangliosides. This ionic liquid matrix offered excellent sensitivity for detection gangliosides without significant loss of sialic acid residues. Thus, it can be used to study the abundance and anatomical localization of gangliosides in mouse brain using mass spectrometry imaging technique. Mass spectrometry image analyses of the mouse brain tissue sections demonstrated that the N-fatty acyl chains of gangliosides were differentially distributed in mouse hippocampal regions, whereby the gangliosides with N-C₁₈ acyl chainwere enriched in CA1 region, while gangliosides with N-C₂₀ acyl chain were enriched in dentate gyrus. In addition, this observation is true for mono-, di- and tri-sialylated gangliosides. Although the linkage information was not determined, the mass spectrometry imaging technique was capable of spatial tissue mapping of ceramide structures in gangliosides.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences
Peer reviewedYes
NPARC number15336775
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Record identifierfba3833a-5acc-43a8-a2d9-45f454e3e737
Record created2010-05-21
Record modified2016-05-09
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