Long-term in vitro human pancreatic islet culture using three-dimensional microfabricated scaffolds

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DOIResolve DOI: http://doi.org/10.1016/j.biomaterials.2010.10.036
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TypeArticle
Journal titleBiomaterials
ISSN0142-9612
Volume32
Issue6
Pages15361542; # of pages: 7
SubjectECM (extracellular matrix); Extracellular matrices; Human islets; In-vitro; Insulin gene; Islet functionality; Long-term culture; Microfabricated; Pancreatic hormones; Stimulation indices; Suspension cultures; Three-dimensional environment; Gene expression; Microfabrication; collagen type 1; biodegradability; extracellular matrix; gene expression; insulin release; microtechnology; pancreas islet; suspension cell culture; three dimensional imaging; Extracellular Matrix; Fluorescent Antibody Technique; Islets of Langerhans; Reverse Transcriptase Polymerase Chain Reaction; Tissue Scaffolds
AbstractHuman pancreatic islet in vitro culture is very challenging and requires the presence of various extra cellular matrix (ECM) components in a three-dimensional environment, which provides mechanical and biological support. The development of such an environment is vital in providing favourable conditions to preserve human islets in long-term culture. In this study, we investigated the effects of human islet culture within various three-dimensional environments; collagen I gel, collagen I gel supplemented with ECM components fibronectin and collagen IV, and microfabricated scaffold with ECM-supplemented gel. The cultured human islets were analyzed for functionality, gene expression and hormone content following long-term in vitro culture. It was clear the incorporation of ECM components within the three-dimensional support improved prolonged culture. However, long-term and highly uniform human islet culture within a microfabricated scaffold, with controlled pore structures, coupled with the presence of ECM components, displayed an insulin release profile similar to freshly isolated islets, yielding a stimulation index of ∼1.8. Moreover, gene expression was markedly increased for all pancreatic genes, giving a ∼50-fold elevation of insulin gene expression with respect to suspension culture. The distribution and presence of pancreatic hormones was also highly elevated. These findings provide a platform for the long-term maintenance and preservation of human pancreatic islets in vitro. © 2010 Elsevier Ltd.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Medical Devices
Peer reviewedYes
NPARC number21271978
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Record identifierfbad267f-46d0-48b8-8501-9304c99896bd
Record created2014-05-15
Record modified2016-05-09
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