Multiplex pathogen detection based on spatially addressable microarrays of barcoded resins

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DOIResolve DOI: http://doi.org/10.1002/biot.200700236
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TypeArticle
Journal titleBiotechnology Journal
ISSN18606768
18607314
Volume3
Issue7
Pages948953; # of pages: 6
SubjectAntigens; barcoded resins; biothreat pathogens; multiplex immunoassays; raman spectroscopy
AbstractSuspension microsphere immunoassays are rapidly gaining recognition in antigen identification and infectious disease biodetection due to their simplicity, versatility and high-throughput multiplex screening. We demonstrate a multiplex assay based on antibody-functionalized barcoded resins (BCRs) to identify pathogen antigens in complex biological fluids. The binding event of a particular antibody on given bead (fluorescence) and the identification of the specific pathogen agent (vibrational fingerprint of the bead) can be achieved in a dispersive Raman system by exciting the sample with two different laser lines. Anthrax protective antigen, Franciscella tularensis lipopolysaccharide and CD14 antigens were accurately identified and quantified in tetraplex assays with a detection limit of 1 ng/mL. The rapid, versatile and simple analysis enabled by the BCRs demonstrates their potential for multiplex antigen detection and identification in a reconfigurable microarray format.
Publication date
PublisherJohn Wiley & Sons
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences; National Institute for Nanotechnology
Peer reviewedYes
NRC number531
565
NPARC number12329039
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Record identifierfc61b995-6c10-4c60-894e-65202f603161
Record created2009-09-10
Record modified2016-05-09
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