Assessment of multidrug resistance on cell coculture patterns using scanning electrochemical microscopy

  1. Get@NRC: Assessment of multidrug resistance on cell coculture patterns using scanning electrochemical microscopy (Opens in a new window)
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Journal titleProceedings of the National Academy of Sciences of the United States of America
Pages92499254; # of pages: 6
Subjectferrocene derivative; ferrocenemethanol; multidrug resistance protein 1; ruthenium complex; unclassified drug; article; cancer cell; cell culture; cell structure; controlled study; electrochemistry; female; HeLa cell; human; human cell; kinetics; multidrug resistance; priority journal; protein localization; quantitative analysis; scanning electrochemical microscopy; target cell; uterine cervix cancer; HeLa cells; microelectrode; MRP1; Cell Culture Techniques; Drug Resistance, Multiple; Female; Ferrous Compounds; HeLa Cells; Humans; Microelectrodes; Microscopy, Fluorescence; Microscopy, Scanning Probe; Multidrug Resistance-Associated Proteins
AbstractThe emergence of resistance to multiple unrelated chemotherapeutic drugs impedes the treatment of several cancers. Although the involvement of ATP-binding cassette transporters has long been known, there is no in situ method capable of tracking this transporter- related resistance at the single-cell level without interfering with the cell's environment or metabolism. Here, we demonstrate that scanning electrochemical microscopy (SECM) can quantitatively and noninvasively track multidrug resistance-related protein 1- dependent multidrug resistance in patterned adenocarcinoma cervical cancer cells. Nonresistant human cancer cells and their multidrug resistant variants are arranged in a side-by-side format using a stencil-based patterning scheme, allowing for precise positioning of target cells underneath the SECM sensor. SECM measurements of the patterned cells, performed with ferrocenemethanol and [Ru(NH3)6]3+ serving as electrochemical indicators, are used to establish a kinetic "map" of constant-height SECM scans, free of topography contributions. The concept underlying the work described herein may help evaluate the effectiveness of treatment administration strategies targeting reduced drug efflux.
Publication date
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270567
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Record identifierfcc2b639-e891-4e35-bed1-4f7e76890995
Record created2014-02-17
Record modified2016-05-09
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